SST PARAMETERS
RETENTION TIME
It is the amount of time taken for a compound (analyte) to pass through the HPLC column and reach the detector after being injected. This is used to find the elution time under specific chromatographic conditions
TAILING FACTOR
It is the measure of peak symmetry in a chromatogram. Ideally, the peaks produced should be symmetrical; they may tail (stretch out on the right side), this can affect accuracy and resolution.
THEORETICAL PLATES
It is the measure of column efficiency and indicates how well the column separates analytes. Higher the plate count better the column’s efficiency, producing sharper, narrower peaks with better resolution.
RELATIVE STANDARD DEVIATION
Relative Standard Deviation(%RSD ) is a statistical measure which is used to evaluate the precision of multiple injections of the same solution (usually a standard). Used to express the variation in peak area, peak height, or retention time as a percentage of the mean. A lower %RSD indicates better precision
SIGNAL-TO-NOISE RATIO
It measures the clarity of a chromatographic peak compared to background noise. This is performed by comparing the height of the analyte peak to the random baseline fluctuations (noise).higher S/N ratio means the peak is more distinguishable from the baseline noise which is essential for detecting low-concentration analytes.