HPLC Blogs
What is HPLC
HPLC or known as High-Performance Liquid Chromatography as full form, is a widely used sophisticated instrument used to separate a mixture of components. Learn about its principles, instrumentation, types of chromatography, and how it helps in chemical and pharmaceutical analysis
How to start HPLC/ HPLC Setup
A beginner-friendly guide to setting up an HPLC system, Perfect for students or new lab analysts. this guide ensures accurate results, and promotes effective operation of High-Performance Liquid Chromatography instruments from the start.
HPLC Mobile Phase
In Hplc mobile phase refers to a liquid solvent (it may be a single solvent or a mixture of solvents) that flows through the Hplc column carrying the sample with it. This guide covers solvent and buffer selection, preparation techniques, filtration, degassing, and mobile phase types.
HPLC Sample
In Hplc, sample refers to the solution that contains the analyte that is to be analyst, for this a proper sample preparation is important for accurate, reproducible, and reliable results.Explore the essentials of HPLC sample preparation and analysis, including sample types, vial selection, filtration, dilution, and injector systems.
System Suitability Test in HPLC
Learn the fundamentals of HPLC System Suitability Testing in this beginner-friendly guide. Understand key parameters like retention time, tailing factor, theoretical plates, and %RSD. Discover how to calculate each parameter, what acceptable limits are, and why they matter for accurate, reliable results before running actual samples in your HPLC analysis.
Method Validation in HPLC
Learn how to check if your HPLC method gives correct and consistent results. This simple guide covers accuracy, precision, specificity, linearity, LOD, LOQ, and more. Designed for beginners, it helps you understand each step of method validation in an easy and practical way.