HPLC Blogs

Free Online HPLC Training Course with Certificate​

Unlock the power of analytical chemistry with our free online HPLC training course! Designed for students, lab professionals, and anyone curious about chromatography, this beginner-friendly course for understanding the basics to running your own virtual HPLC analysis. you can learn anytime, anywhere and earn a certificate upon completion.

What is HPLC

HPLC or known as High-Performance Liquid Chromatography as full form, is a widely used sophisticated instrument used to separate a mixture of components. Learn about its principles, instrumentation, types of chromatography, and how it helps in chemical and pharmaceutical analysis

How to start HPLC/ HPLC Setup

A beginner-friendly guide to setting up an HPLC system, Perfect for students or new lab analysts. this guide ensures accurate results, and promotes effective operation of High-Performance Liquid Chromatography instruments from the start.

HPLC Mobile Phase

In Hplc mobile phase refers to a liquid solvent (it may be a single solvent or a mixture of solvents) that flows through the Hplc column carrying the sample with it. This guide covers solvent and buffer selection, preparation techniques, filtration, degassing, and mobile phase types. 

HPLC Sample

In Hplc, sample refers to the solution that contains the analyte that is to be analyst, for this a proper sample preparation is important for accurate, reproducible, and reliable results.Explore the essentials of HPLC sample preparation and analysis, including sample types, vial selection, filtration, dilution, and injector systems.

Learn HPLC Online – Free Training

Learn HPLC with Assayprism’s free online training. Designed for beginners, it offers flexible, self-paced learning to build real lab skills. Start anytime, enhance your career, and gain confidence in chromatography without needing access to expensive lab equipment or lab space.

System Suitability Test in HPLC

Learn the fundamentals of HPLC System Suitability Testing in this beginner-friendly guide. Understand key parameters like retention time, tailing factor, theoretical plates, and %RSD. Discover how to calculate each parameter, what acceptable limits are, and why they matter for accurate, reliable results before running actual samples in your HPLC analysis.

Method Validation in HPLC

Learn how to check if your HPLC method gives correct and consistent results. This simple guide covers accuracy, precision, specificity, linearity, LOD, LOQ, and more. Designed for beginners, it helps you understand each step of method validation in an easy and practical way.

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