HPLC LEARNING PLATFORM
TABLETS
Level 1 – Single Drug (Compound A)
This beginner-level module introduces basic HPLC analysis using a single compound (Compound A). It covers simple sample preparation, methanol as the mobile phase, and a straightforward isocratic method. Ideal for learners new to HPLC, focusing on fundamental concepts like purity calculation, standard comparison, and essential lab equipment handling.
Level 2 – Single Drug (Compound B)
Building on Level 1, this procedure introduces buffer preparation, pH adjustment, and dual-solvent systems using phosphate buffer and acetonitrile. It adds centrifugation for improved sample clarity. Still single-analyte, Level 2 helps students understand slightly more advanced solvent systems and sample prep techniques in realistic, but controlled, learning conditions.
Level 3 – Multi-Drug (Compounds C & D)
This intermediate module involves quantification of two compounds in one tablet. Learners prepare combined standards and sample solutions, analyze them in a single HPLC run. It introduces binary mobile phases, dual retention peaks, and multicomponent calculations, enhancing understanding of combination drug analysis and chromatographic separation challenges.
Level 4 – Multi-Drug (Compounds E & F)
Level 4 introduces gradient elution for separating multiple compounds with differing polarities. It deepens understanding of gradient programming, buffer-acetonitrile ratios, and precision in timing. This level is suited for students familiar with HPLC basics who are ready for professional-level multicomponent method development and analysis.
Important Note:
The compounds referred to as Compound A, B, C, D, E, and F in these procedures are entirely fictional and are used solely for educational and demonstration purposes. They do not represent any real pharmaceutical substances. All methods and values are simplified to support learning and should not be applied in real laboratory or clinical settings.